Journal: Nature Microbiology

Article Title: Uropathogenic Escherichia coli invade luminal prostate cells via FimH–PPAP receptor binding

doi: 10.1038/s41564-025-02231-0

Figure Lengend Snippet: a – c , Representative confocal microscopy images of recombinant FimH LD (rFimH LD ) binding to the 5637 bladder cell line ( a ), organoid-based model grown with 10 nM DHT ( b ) and human prostate tissue ( c ). rFimH LD signal (anti-FLAG antibody) is shown in red. Nuclei were counterstained using Hoechst 33342 (blue). Scale bar, 25 µm ( n = 3 biological replicates). d , Overlay assay: prostate, 5637 and the rFimH LD -FLAG protein samples were resolved in an SDS–PAGE, transferred to PVDF membrane and incubated with the rFimH LD -FLAG in the presence or absence of 2.5% of ᴅ -mannose. Prostate protein candidates were detected using an anti-FLAG antibody. The two most prominent bands were excised and identified as PPAP and DDAH1. n = 2 biological replicates. e , Co-immunoprecipitation of PPAP using rFimH LD -FLAG as bait ( n = 3 biological replicates). f , g , Representative blot ( f ) and quantification ( g ) of rFimH LD -FLAG binding to rPPAP-HA WT or mutants (mutant 1 position 94, mutant 2 position 220 and mutant 3 position 333; fold change over PPAP-HA signal; n = 3 biological replicates, one-way ANOVA with Dunnett’s post hoc test). h , Binding array showing UTI89 WT or UTI89 Δ fimH mutant binding to rPPAP-HA (WT and the triple mutant 1+2 +3). Mock-transfected (MT) cell lysate was used as control ( n = 3 biological replicates, one-way ANOVA with Tukey’s post hoc test). Data in g and h are presented as mean ± s.d.

Article Snippet: To confirm that rFimH LD could bind to host cells, we first used the 5637 cell line (HTB-9, ATCC) as control.

Techniques: Confocal Microscopy, Recombinant, Binding Assay, Overlay Assay, SDS Page, Membrane, Incubation, Immunoprecipitation, Mutagenesis, Transfection, Control

Journal: NPJ Precision Oncology

Article Title: ACTG1 driven splicing of P4HB gene enhances EMT and bladder cancer progression

doi: 10.1038/s41698-025-00923-8

Figure Lengend Snippet: A Upset plot of AS events in BLCA. B Unisex Upset plot illustrating adverse events related to AS in BLCA, combined with clinical information.

Article Snippet: The human BLCA cell lines T24 and RT4 were cultured in McCoy’s 5a medium (catalog number: 30-2007, ATCC) containing 10% fetal bovine serum, while human T24-Luc cells and human BLCA cell line 5637 were cultured in RPMI-1640 medium (catalog number: 30-2001, ATCC) also containing 10% fetal bovine serum.

Techniques:

Journal: NPJ Precision Oncology

Article Title: ACTG1 driven splicing of P4HB gene enhances EMT and bladder cancer progression

doi: 10.1038/s41698-025-00923-8

Figure Lengend Snippet: A Schematic diagram showing the variation of AS events with penalty coefficient in LASSO analysis. B Schematic diagram for calculating the optimal penalty parameter λ . The x -axis represents the logarithmic ( λ ) values and the y -axis represents the degrees of freedom. The λ values selected based on the minimum criteria and the 1-SE criterion resulted in 9 AS events. C Expression trend of ACTG1 in normal and BLCA patients. D Kaplan–Meier survival curves for high and low expression groups of ACTG1. ** Indicates a significant difference between the two groups with P < 0.01, Normal (blue) = 19, Tumor (red) = 407.

Article Snippet: The human BLCA cell lines T24 and RT4 were cultured in McCoy’s 5a medium (catalog number: 30-2007, ATCC) containing 10% fetal bovine serum, while human T24-Luc cells and human BLCA cell line 5637 were cultured in RPMI-1640 medium (catalog number: 30-2001, ATCC) also containing 10% fetal bovine serum.

Techniques: Expressing

Journal: NPJ Precision Oncology

Article Title: ACTG1 driven splicing of P4HB gene enhances EMT and bladder cancer progression

doi: 10.1038/s41698-025-00923-8

Figure Lengend Snippet: A Forest plot comparing the expression of ACTG1 between BLCA and Normal groups. B Forest plot comparing the expression of ACTG1 in subgroups (Country) of BLCA and Normal groups. C and D Funnel plots for assessing publication bias and verification using trim-and-fill method. E Sensitivity analysis using leave-one-out approach; MD denotes mean difference, and 95% CI represents a 95% confidence interval.

Article Snippet: The human BLCA cell lines T24 and RT4 were cultured in McCoy’s 5a medium (catalog number: 30-2007, ATCC) containing 10% fetal bovine serum, while human T24-Luc cells and human BLCA cell line 5637 were cultured in RPMI-1640 medium (catalog number: 30-2001, ATCC) also containing 10% fetal bovine serum.

Techniques: Expressing

Journal: NPJ Precision Oncology

Article Title: ACTG1 driven splicing of P4HB gene enhances EMT and bladder cancer progression

doi: 10.1038/s41698-025-00923-8

Figure Lengend Snippet: A Tumor immune cell infiltration in BLCA patients grouped by ACTG1 expression levels. B – I Correlation of ACTG1 with 8 types of immune cells. J ssGSEA scores of BLCA patient samples classified based on low or high ACTG1 expression levels. K Expression of 20 immune checkpoint-related genes in tumors of BLCA patients grouped by ACTG1 expression levels. *Indicates statistical significance between groups, P < 0.05, **Indicates statistical significance between groups, P < 0.01. *** Indicates statistical significance between groups, P < 0.001; Low (in blue) represents the low ACTG1 expression group, High (in red) represents the high ACTG1 expression group.

Article Snippet: The human BLCA cell lines T24 and RT4 were cultured in McCoy’s 5a medium (catalog number: 30-2007, ATCC) containing 10% fetal bovine serum, while human T24-Luc cells and human BLCA cell line 5637 were cultured in RPMI-1640 medium (catalog number: 30-2001, ATCC) also containing 10% fetal bovine serum.

Techniques: Expressing

Journal: NPJ Precision Oncology

Article Title: ACTG1 driven splicing of P4HB gene enhances EMT and bladder cancer progression

doi: 10.1038/s41698-025-00923-8

Figure Lengend Snippet: A – C Volcano plots showing the DEcircRNAs in BLCA tumor tissue samples compared to normal tissue samples from three datasets: GSE92675 (tumor = 4, normal = 4), GSE97239 (tumor = 3, normal = 3), and GSE147985 (tumor = 4, normal = 4). D Intersection of DEGs in the three datasets.

Article Snippet: The human BLCA cell lines T24 and RT4 were cultured in McCoy’s 5a medium (catalog number: 30-2007, ATCC) containing 10% fetal bovine serum, while human T24-Luc cells and human BLCA cell line 5637 were cultured in RPMI-1640 medium (catalog number: 30-2001, ATCC) also containing 10% fetal bovine serum.

Techniques:

Journal: NPJ Precision Oncology

Article Title: ACTG1 driven splicing of P4HB gene enhances EMT and bladder cancer progression

doi: 10.1038/s41698-025-00923-8

Figure Lengend Snippet: A Venn diagram showing the intersection of genes encoding DEcircRNAs and EMT-related genes. B Correspondence graph between EMT-related genes and their encoding DEcircRNAs. C – E Expression trends of EMT-related genes encoding DEcircRNAs in normal and BLCA patients; *** indicates P < 0.001 compared between the two groups; Normal (blue) = 19, Tumor (red) = 407.

Article Snippet: The human BLCA cell lines T24 and RT4 were cultured in McCoy’s 5a medium (catalog number: 30-2007, ATCC) containing 10% fetal bovine serum, while human T24-Luc cells and human BLCA cell line 5637 were cultured in RPMI-1640 medium (catalog number: 30-2001, ATCC) also containing 10% fetal bovine serum.

Techniques: Expressing

Journal: NPJ Precision Oncology

Article Title: ACTG1 driven splicing of P4HB gene enhances EMT and bladder cancer progression

doi: 10.1038/s41698-025-00923-8

Figure Lengend Snippet: Schematic representation of the molecular mechanism by which the variable shear regulatory factor ACTG1 regulates the splicing of EMT-related genes to generate circRNAs, promoting the growth and metastasis of BLCA.

Article Snippet: The human BLCA cell lines T24 and RT4 were cultured in McCoy’s 5a medium (catalog number: 30-2007, ATCC) containing 10% fetal bovine serum, while human T24-Luc cells and human BLCA cell line 5637 were cultured in RPMI-1640 medium (catalog number: 30-2001, ATCC) also containing 10% fetal bovine serum.

Techniques: Shear